The enormous rise in energy demand with the strict emissions regulations has put together in search of the finest alternative fuel that meets the various emission norms of the vehicles made worldwide. Nowadays, researchers all around the globe aims to reduce the amounts of emissions from the compression ignition (CI) engine. Biofuel resembles the qualities of fossil diesel while it is also proven to be a greener fuel and viable alternative. However, the primary challenge of using biodiesel was higher emissions of Nitrogen oxides (NOx). Hence to minimize the NOx, an exhaust gas recirculation (EGR) system could be utilized which could resolve the challenges associated with this higher NOx emissions. In this study, juliflora biodiesel was selected and blended with diesel at 20% by volume (B20) and three different EGR percentages 5%, 10%, and 15%, were investigated on the characteristics of CI engine. Based on the test outcomes, a drop in NOx has been observed with a 15% rate of EGR which
is 6.1% lower than conventional diesel. Moreover, brake thermal efficiency increased by 11% as compared to diesel. However, a slight increase in other exhaust emissions was noticed with EGR. The results conclude that, lower rate of EGR (15%) with B20 blend of juliflora biodiesel provides the optimum performance in the engine with least NOx emission.