The designation of this research is to scrutinize the influence of convective nanofluids over a three-dimensional exponential surface with chemical reactive species in a free stream fluid flow by following Buongiorno’s model. The continuity, momentum, energy, concentration and motile microorganism density partial differential equations that make up the physical governing equation problems are simultaneously transformed into ordinary differential equations system. By using MATLAB programming, the RKF approach has been followed in order to implement the shooting technique to solve this system that explores how changing fluid parameters affect the profile of physical quantities of interest. A parametric analysis has been done in the current study. The effects of fluid parameters such as chemical reaction, Brownian motion, free stream velocity, Lewis number, thermophoresis, and Prandtl number on concentration, temperature, and velocity profiles are graphically represented. Moreover, Contour plots are also drawn against computational fluid parameters to get desired results. Furthermore, calculated results are correlated with already existing outcomes along with residual error. It is inferred that; thermal and concentration fields increase for higher thermal and concentration Biot numbers serially. Additionally, it is found that skin friction coefficient declines with inclination in thermophoresis Nt (1.0 ≤ Nt ≤ 3.0) and Prandtl number Pr (1.0 ≤ Pr ≤ 4.0). The present investigation aims to support production businesses in achieving the desired level of quality of their products by effectively managing the transport phenomena.