The Organic Rankine Cycle (ORC) is a promising technology for power generation from low-grade heat. The selection of working fluids is one of the important key points to improve the performance of an ORC system. Zeotropic mixtures show promising performances as working fluids. In fact, their temperature glide during phase change enables better match between the working fluid and the heat source/sink temperatures. In order to reveal the performance of mixture in ORC system, this paper deals with the thermodynamic model of the subcritical Organic Rankine Cycle (ORC) systems driven by low grade heat source while using zeotropic mixture working fluids with a special consideration to the interaction between phase change glides and the pinch value and their location in both the evaporator and the condenser (HEXs). Zeotropic mixtures of seven pure fluids are evaluated as working fluids for a subcritical ORC system. The mass fraction effects of mixtures on the thermal efficiency are analyzed. For given working conditions (working fluid mass flow, pressure and bubble temperature) the results show that for each considered zeotropic mixture there exist mass fraction ranges that are not consistent with the pinch values constraint in the HEXs and leads to so-called ‘infeasible zones’ with unreal HEXs dimensions. Results shows also that, out of these “infeasible fractions” zone, keeping unchanged the working conditions, the thermal performances of ORC system using zeotropic mixture are always better than the thermal performances of the same systems using the correspondent pure fluids. In addition, out of these highlighted “unfeasible zones” it was found that mixture with high temperature glide improve the thermal efficiency of ORC system.