COVID-19 virus, which is a member of the coronavirus family, is a virulent virus that is transmitted by physical contact or air and results in death in infected people. People infected by the COVID-19 virus must be kept in quarantine in a closed area. Therefore, it is very important for the health of the individuals (patient, doctor, nurse, etc.) in the isolation room that, where the area kept closed is completely free of virus. This study was conducted to examine the flow dynamics of the air in the isolation chamber. With the simultaneous operation of the sanitized air machine and the ventilation in the room, it is aimed to investigate the virus killing effect of the air mixed with each other. The flow dynamics, velocity, temperature and turbulence kinetic energy of the air in the isolation chamber were examined. Investigations were carried out to ensure that the mixture of the two air in the room was better and distributed throughout the room.