In this paper comparative analysis of R152a, R600, R600a, R410a, R290, R1234yf, R404a and R134a as refrigerants in two stage vapour compression refrigeration system has been done on the basis of energetic and exergetic performance. Performance parameters such as entropy generations, COP, exergetic efficiency, sustainability index were investigated at different ambient condition. It was found that both energy and exergy efficiencies of R134a is 8.97% and 5.38% lower than R152a and R600 respectively at -50 oC evaporating and 45 oC condensing temperatures. It was also observed that Irreversibility was minimal at higher evaporating temperatures and condenser was responsible for highest irreversibility or losses in two stage vapour compression refrigeration system. Sustainability index for R152a (1.96) was highest compared to other refrigerants.