Axoplasmic fluid properties for neuronal excitation have been investigated with respect to temperature. Density, the mass fraction of ions and rate of addition of ions are the parameters considered for characterizing axoplasmic fluid properties. The behavi or of these parameters has been analyzed with respect to the changes in temperature ranging from 5 degree Ce lsius to 35 degree Celsius. The temp erature has been defined using Q 10of3 coefficient as done in the Hodgkin Huxley model. The trend of these param eters at different temperatures has been depicted along the axonal length represented through x axis of the graphs. The conduction velocities of the above said parameters have also been recorded at diff erent temperatures. The range [−5,35] degree C elsius has been increased by 20 degrees, 10 degree on the lower side and 10 degree on the upper side of the ra nge [−5,25] degree Celsius and it is found that temperature dependency using Q 10of3 coefficient for said parameters is valid only i n the temperature ranging from 5 degree Celsius to 25 degree Celsius as it is for membrane voltage in the Hodgkin Huxley model . These findings strongly support the obtained results and also suggest obtaining the temperature coefficient value which is appli cable for a wider range of temperatures impacting neuronal excitation.